What are the standard calibration ranges for Reb M HPLC analysis?
GB1886.355-2022 specifies calibration using 100-1000mg/L standards for routine analysis, with detection limit of 100mg/kg. Sample injection volume is 2-10μL using C18 column (4.6×150mm, 5μm) at 210nm detection.
How does Sinopure ensure Reb M raw material quality?
Sinopure controls 10,000 acres of stevia farms in China (Anhui, Henan, Jiangsu, Gansu), producing 5,000 tons of premium dried leaves annually. Leaves are processed within 8 hours of harvest with moisture ≤10% to maintain glycoside content.
Why is Reb M preferred for pharmaceutical applications?
With zero calories and excellent stability (≤3.2% degradation at 180°C), Reb M effectively masks bitter drug flavors in tablets and protein powders while meeting GB1886.355-2022 pharmaceutical excipient requirements.
What temperature conditions are required for Reb M sample preparation?
GB1886.355-2022 specifies 80°C extraction for 30 minutes using methanol/water (7:3 v/v) solution for optimal recovery. HPLC analysis requires column temperature at 40°C with 0.8mL/min flow rate for accurate quantification.
What are Reb M’s pharmaceutical applications?
Reb M is used in tablets, protein powders, and pet foods due to zero-calorie property and clean taste. It masks bitter flavors in medicines while meeting GB1886.355-2022 safety standards for pharmaceutical excipients.
What makes Reb M superior for premium beverage applications?
Reb M provides clean sweetness without bitter aftertaste, making it perfect for high-end beverages. With 500× sucrose sweetness intensity and excellent stability in acidic conditions, it outperforms other sweeteners in clarity and taste profile.
How is Reb M extracted and purified industrially?
Sinopure utilizes a five-stage membrane separation system combined with continuous chromatography to produce 200 tons/year of high-purity Reb M (≥98%). The process achieves 92% recovery rate while maintaining strict GB1886.355-2022 standards.
What is the HPLC detection method for Reb M purity?
According to GB1886.355-2022, use C18 column (4.6×150mm, 5μm) with 0.8mL/min flow rate and 210nm detection wavelength. The mobile phase requires 25-45% acetonitrile gradient in 0.1% phosphoric acid for baseline separation.
What quality certifications are available for Reb M?
Our monk fruit powder products meet international standards including FDA GRAS, ISO9001, ISO22000, FSSC22000, HALAL, and KOSHER certifications, ensuring global market compliance.