Reb D Series – Natural Sweetener Solutions

What microbial standards apply to Reb D powder?

USP mandates: TAMC ≤1000 CFU/g, TYMC ≤100 CFU/g, absence of E. coli in 10g. Our irradiation treatment (5kGy gamma) achieves sterility assurance level 10⁻³ while causing

How does HPLC column selection differ for Reb D analysis?

Zorbax SB-C18 (3.5μm, 4.6×150mm) provides 1.8x better resolution than standard C18 for Reb D/Reb M separation (Rs=2.3). Mobile phase requires 22% acetonitrile in 0.1% phosphoric acid (v/v) at 1.2mL/min for optimal peak symmetry (As=0.95-1.05).

What membrane pore size is optimal for Reb D ultrafiltration?

10kDa ceramic membranes achieve 98.5% Reb D recovery while removing 99.2% of pigments and proteins. Operating at 2.5bar transmembrane pressure with 50°C feed temperature maintains flux rate of 15-18LMH without fouling.

Does Reb D exhibit different crystallization behavior than Reb A?

Reb D requires 12-15% slower cooling rates (0.2°C/min vs 0.35°C/min for Reb A) due to its anisotropic crystal growth. Orthorhombic crystals form at 55-58% ethanol concentration, yielding 20% larger particle size (150-180μm) with superior purity (99.3±0.4%).

Does Reb D require different pasteurization parameters than Reb A?

Yes, Reb D solutions tolerate higher temperatures: 85°C for 40s (vs 75°C for Reb A) due to its glycosylation pattern. This allows for commercial sterilization while maintaining

What’s the pH stability range for Reb D solutions?

Reb D demonstrates superior pH stability vs Reb A, with

How does Reb D perform in high-Brix systems?

Reb D shows 23% less sweetness suppression than Reb A in 65°Brix solutions, with linear sweetness perception up to 500ppm concentration. Solubility remains >100g/L in 60°Brix sucrose solutions at 4°C, making it ideal for concentrated beverage syrups.

What particle size distribution is ideal for Reb D powder?

Optimal Reb D powder achieves D50=120-150μm with span value ≤1.8 through jet milling (2.5 bar, -20°C). This ensures bulk density of 0.45-0.55g/cm³ for optimal flowability while maintaining dissolution time ≤15s in 25°C water.

How is color controlled in Reb D final product?

Activated carbon treatment (0.5% w/v, 60°C for 30min) achieves APHA color ≤20. Hunter Lab values must show L* ≥85, a* -0.5 to +0.5, b* ≤5.0, measured against 10% aqueous solution with spectrophotometer at 420nm.

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